A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions, transparent or opaque solids, such as polished glass, or gases. In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.
Ultraviolet spectrophotometer is an effective method to study the composition, structure and interaction of substances according to the absorption spectrum of substances.
Spectrophotometry is often used in measurements of enzyme activities, determinations of protein concentrations, determinations of enzymatic kinetic constants, and measurements of ligand binding reactions.
With the increasing emphasis on quality work, quality control is more from empirical control to parameter control, and the use of atomic absorption spectrophotometers has become increasingly widespread.